RNA detection based on cyclic amplification of graphene oxide fluorescence

Y.-H. Wu, C.-M. Jiang, G.-X. Yang

Linyi People’s Hospital Affiliated to Shandong University School of Medicine, Shandong, China. v96296@sina.com

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• Oncology

OBJECTIVE: Graphene oxide (GO) was prepared and used to adsorb single-stranded DNA. Based on fluorescence quenching ability of GO, cyclic signal amplification was performed using polymerase. Thus, two RNA sequences were detected by fluorescence assay.

METHODS: Klenow fragment was designed for the amplification of fluorescence signals and RNA was detected by fluorescence assay. After amplification with Klenow fragment, the lowest limit of detection was 1.0 × 10-11 M, and the response of fluorescent intensity was linear within the concentration range of 1.0 × 10-11 M -1.0 × 10-9 M. By the modification of DNA terminals with different fluorescent groups, two different RNA were detected.

RESULTS: Different fluorescent dyes were used to modify the terminals of DNA, and two RNA sequences were detected based on fluorescence. There was no need for product separation and purification before detection.

CONCLUSIONS: Detection of RNA based on cyclic amplification of GO fluorescence is fast and simple. It can be used for the analysis of specific RNA sequences in cancer cells.

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