Analysis of expression of cyclin E, p27kip1 and Ki67 protein in colorectal cancer tissues and its value for diagnosis, treatment and prognosis of disease

W. Li, G. Zhang, H.-L. Wang, L. Wang

The Pain Department of Cangzhou Central Hospital, Cangzhou City, Hebei Province, China. hai_lunwang1@163.com

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• Oncology

OBJECTIVE: We conducted this study is to investigate the clinical application value of Cyclin E, p27kip1 and Ki67 protein expression in colorectal cancer tissues for diagnosis, treatment, and prognosis of this disease.

PATIENTS AND METHODS: The positive expression of Cyclin E, p27kip1 and Ki-67 in tissues of 200 patients with colorectal cancer and 200 patients with benign colorectal tumor or inflammation were detected by immunohistochemistry PowerVision two-step method. RT-PCR was used to detect the expression level of the corresponding mRNA, as well as to analyze the association with TNM staging, pathology type, free progression survival and median survival. The sensitivity, specificity, and accuracy of diagnosis were analyzed by ROC.

RESULTS: The positive expression rate and positive degree of Cyclin E and Ki-67 of observation group were higher than those of the control group, while positive expression rate and positive degree of p27kipl was lower than that of the control group; the differences were statistically significant (p<0.05). The quantitative expression levels of Cyclin E and Ki-67 mRNA of observation group were distinctly higher than those of the control group, while p27kipl was evidently lower than that of the control group; the differences were statistically significant (p<0.05). With the increase of TNM staging, the positive expression of Cyclin E and Ki-67 increased, p27kipl decreased, and the difference was statistically significant (p<0.05). With the decrease of differentiation degree, the positive expression of Cyclin E and Ki-67 increased, p27kipl decreased, and the difference was statistically significant (p<0.05). The free progression survival and median survival of positive expression and negative expression of Cyclin E and Ki-67 were shortened, p27kipl extended, and the difference was statistically significant (p<0.05). The diagnostic sensitivity of Cyclin E mRNA was 89.6%, specificity 84.5%, accuracy 0.823 and the critical value was 0.3562; The diagnostic sensitivity of p27kipl mRNA was 80.5%, specificity 76.5%, accuracy 0.802 and the critical value was 0.3023. The diagnostic sensitivity of Ki-67 mRNA was 86.5%, specificity 82.9%, accuracy 0.814 and the critical value was 0.3243.

CONCLUSIONS: We discovered that Cyclin E and Ki67 protein expression of colorectal cancer tissues was upregulated and p27kipl protein expression was downregulated, which were closely related to the TNM and pathological differentiation degree. These values were also closely associated with free progression survival and median survival of prognosis. Therefore, the above indexes can be used as highly sensitive, specific and accurate markers for the diagnosis of colorectal cancer.

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