MiR-630 promotes epithelial ovarian cancer proliferation and invasion via targeting KLF6

S. Zhang, J.-Y. Zhang, L.-J. Lu, C.-H. Wang, L.-H. Wang

Department of Gynecology, Zhangjiagang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Zhangjiagang, Jiangsu Province, China. 1401364281@qq.com

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• Oncology

OBJECTIVE: MicroRNAs play critical roles in post-translational gene expression. The current study was to investigate the effects of miR-630 in epithelial ovarian cancer.

PATIENTS AND METHODS: Thirty epithelial ovarian cancer tissue and thirty normal ovarian tissue samples were collected and were detected miR-630 expression level with qRT-PCR. MiR-630 mimics, inhibitors and negative controls were transfected into SKOV3 and Cell Counting Kit-8 (CCK-8) assay, and transwell experiment were performed to detect the proliferation rate and migration, respectively. The luciferase reporter assay was utilized to identify miR-630’s target gene. Balb/c nude mice were utilized to verify the effect of miR-630 in vivo.

RESULTS: QRT-PCR showed a significantly high miR-630 expression in epithelial ovarian cancer relative to normal ovarian tissue. The miR-630 overexpression promoted epithelial ovarian cancer cell SKOV3 proliferation and migration. Krüppel-like factor 6 (KLF6) was predicted as the target of miR-630. In vivo study also verified that miR-630 overexpression stimulated ovarian cancer growth.

CONCLUSIONS: We propose that targeting miR-630 might be a promising therapeutic approach for ovarian cancer.

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