A novel approach to study oxidative stress in thyroid diseases: a preliminary study

A. Metere°*, C. Chiesa*, C. Di Cosimo*, G. Fierro#, L. Giacomelli*, D. Pietraforte°

°Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Rome (Italy)
*Department of Surgical Sciences, “Sapienza” University of Rome (Italy)
#Department of Cardiovascular Respiratory Nephrological and Geriatric Sciences, “Sapienza” University of Rome (Italy)

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• Endocrinology

BACKGROUND AND OBJECTIVES: Recently, several Authors have emphasized the relationship between oxidative stress and thyroid tumors. Several methods have been proposed in the literature for the measurement of oxidative stress in human tissues, although the high reactivity and short half life of Reactive Oxygen and Nitrogen Species make difficult their direct determination. Here we propose a novel approach for the determination of oxidative stress in human tissues, taking into account the relationship between free radicals and thyroid diseases.

MATERIALS AND METHODS: Our goal in this preliminary study, was to demonstrate the opportunity to use the coupling of the EPR-spin trapping technique with the hydroxylamine 1-hydroxy-3-carboxy-pyrrolidine, to detect oxidative stress in the human blood of patients with thyroid disease.

RESULTS: Our preliminary findings confirm that this is a sensible, precise and valid method to study the oxidative stress and encourage us to continue the project.

CONCLUSIONS: Our next goal will be to enroll patients affected by different thyroid diseases and to study the effect of some antioxidants in the management of the disease. This will allow to better understand the pathological path that binds the formation of reactive oxidizing species to the thyroid cancer and eventually to take into account the antioxidant therapy, as a possible additional “therapeutic weapon”.

Corresponding Author: Alessio Metere, MD; e-mail: alessio.metere@iss.it