Eur Rev Med Pharmacol Sci 2018; 22 (9): 2680-2687
DOI: 10.26355/eurrev_201805_14964

MiR-1275 promotes non-small cell lung cancer cell proliferation and metastasis by regulating LZTS3 expression

J. He, L. Yu, C.-M. Wang, X.-F. Zhou

Department of Pneumology, the First Affiliated Hospital of Chengdu Medical College, Chengdu, China. qq157110097@aliyun.com


OBJECTIVE: To detect the expressions of micro-ribonucleic acid 1275 (miR-1275) in non-small cell lung cancer (NSCLC) tissues and cells, analyze the correlations of the expression of miR-1275 with the clinicopathological features of NSCLC, and explore its biological function and potential molecular mechanism.

PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of miR-1275 in NSCLC tissues and cells. The correlations of miR-1275 expression with clinicopathological features of NSCLC were statistically analyzed. Clone formation assay, flow cytometry and transwell assay were used to detect the effects of interfering in miR-1275 expression on biological behaviors of NSCLC cells. Bioinformatics was used to predict the downstream target genes of miR-1275, and qRT-PCR and Western blotting were utilized for verification. Dual luciferase reporter gene assay was used to validate the binding of miR-1275 to its target gene.

RESULTS: The results of qRT-PCR showed that among 70 cases of tissues from NSCLC patients, 52 cases had up-regulated miR-1275 expressions. MiR-1275 expression was increased in NSCLC cells compared with that in 16 human bronchial epithelial (16HBE) cells. Interfering in miR-1275 expression could inhibit the proliferation, invasion and metastasis of NSCLC cells. Bioinformatics prediction discovered that leucine zipper putative tumor suppressor 3 (LZTS3) might be a target gene of miR-1275. Dual luciferase reporter assay confirmed that the two genes could bind directly.

CONCLUSIONS: MiR-1275 is relatively highly expressed in NSCLC. Highly expressed miR-1275 can promote the proliferation and metastasis of NSCLC through regulating the expression of LZTS3.

This article has been retracted. The Publisher apologizes for any inconvenience this may cause.

Free PDF Download

To cite this article

J. He, L. Yu, C.-M. Wang, X.-F. Zhou
MiR-1275 promotes non-small cell lung cancer cell proliferation and metastasis by regulating LZTS3 expression

Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 9
Pages: 2680-2687
DOI: 10.26355/eurrev_201805_14964