Effect of Deltex-1 on proliferation and differentiation of bone marrow mesenchymal stem cells into smooth muscle cells

Y. Wang, B.-P. Yang, Y.-G. Chi, L.-B. Liu, L. Lei

Department of Gynecology and Obstetrics, Chongqing Maternal and Child Health-Care Hospital, Chongqing, China. leili0112@163.com

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• Urology

OBJECTIVE: To investigate the effect of Deltex-1 on proliferation and differentiation of bone marrow mesenchymal stem cells (bMSCs) into smooth muscle cells (SMCs).

MATERIALS AND METHODS: bMSCs of rat were isolated from bone marrow, cultured and identified. The effect of Deltex-1 on the proliferation of bMSCs infected with adenovirus vector pAd/Deltex-1 was detected by cell count kit-8 (CCK-8). The expression of smooth muscle myosin heavy chain (SM-MHC), that is one of the markers of SMCs in bMSCs without treatment, with Deltex-1 virus infection or empty virus infection and co-cultured with SMCs, were detected by immunofluorescence cytochemistry staining, RT-PCR and Western blotting. The same detection of bMSCs and SMCs without treatment was used as normal control, respectively.

RESULTS: bMSCs of rat were isolated from bone marrow, cultured and identified. Compared with the control, the results of CCK-8 showed that the growth of bMSCs infected by Deltex-1 virus was slower, and began to appear more significant especially at 48 (p<0.05, p<0.01). The results of immunofluorescence cytochemistry, Real-time PCR and Western blot showed that bMSCs with Deltex-1 virus infection and co-cultured with SMCs significantly expressed SM-MHC, and weakly expressed Notch-1.

CONCLUSIONS: The proliferation of bMSCs with Deltex-1 over-expression could be inhibited and its differentiation into smooth muscle cells could be promoted.

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