The role of miR-144/GSPT1 axis in gastric cancer

Q.-G. Tian, R.-C. Tian, Y. Liu, A.-Y. Niu, J. Zhang, W.-F. Gao

Department of General Surgery, The Fourth Affiliated Hospital of Baotou Medical College, Baotou, China. 2311030163@qq.com

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• Oncology

OBJECTIVE: To investigate the potential effects of miR-144/GSPT1 axis on the development of gastric cancer.

PATIENTS AND METHODS: The expressions of GSPT1 (G1 to S Phase Transition 1) and miR-144 were detected in gastric cancer tissues and the adjacent normal tissues. We also explored the levels of GSPT1 and miR-144 in both normal gastric cell line (GES-1) and gastric cells (SGC7901). Luciferase assay was conducted to evaluate the interaction between miR-144 and GSPT1. The effects of the miR-144/GSPT1 axis on SGC7901 cells were determined via investigating cell proliferation, invasion and metastasis.

RESULTS: miR-144 was found to be down-regulated in gastric cancer tissues while GSPT1 expression level was markedly increased. Bioinformatics analysis showed that GSPT1 was a direct target of miR-144. Luciferase assays confirmed our hypothesis. The subsequent experiments showed that miR-144 could promote cell proliferation, invasion and migration in gastric cancer cells via inhibiting GSPT1.

CONCLUSIONS: We showed that miR-144/GSPT1 axis could be a potential therapeutic target in treatment of gastric cancer.

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