MicroRNA-199a-5p regulates glioma progression via targeting MARCH8

C. Zhang, Q. Chen, J.-W. Zhu, Z.-F. Liu

Department of Neurosurgery Ward 1, Hanzhong Central Hospital, Hanzhong, P.R. China. liuzhenfeng_xy@126.com

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• Oncology

OBJECTIVE: MicroRNA-199a-5p (miR-199a-5p) was reported to play crucial roles in cancer progression. However, its role in glioma remains largely unknown.

MATERIALS AND METHODS: RT-qPCR was employed to analyze miR-199a-5p expression level in glioma cell lines. The effects of miR-199a-5p on cell proliferation and invasion were investigated in vitro.

RESULTS: We showed that miR-199a-5p expression level was significantly downregulated in glioma cell lines compared with a normal cell. In addition, miR-199a-5p overexpression suppresses glioma cell proliferation and invasion in vitro. Bioinformatic analysis and Luciferase assay suggested that the membrane-associated ring-CH-type finger 8 (MARCH8) was a target of miR-199a-5p. Furthermore, MARCH8 overexpression could partially reverse the effects of miR-199a-5p on glioma cells.

CONCLUSIONS: These findings suggested that miR-199a-5p may function as a tumor suppressor in glioma by targeting MARCH8.

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