Eur Rev Med Pharmacol Sci 2020; 24 (2): 735-739
DOI: 10.26355/eurrev_202001_20053

Effect of miR-200c on migration and proliferation of breast cancer MDA-MB-231 cells and BT-549 cells and the possible mechanism

Y. Lei, Y. Ma, Y. Liu, X.-F. Wang

Department of Medical Oncology, Shaanxi Provincial People’s Hospital, Xi’an, P.R. China. rzkqa7@163.com


OBJECTIVE: This study was designed to investigate the effects of miR-200c on the migration and proliferation of breast cancer MDA-MB-231 cells and BT-549 cells and the possible mechanisms.
MATERIALS AND METHODS: The effects of miR-200c on the proliferation and migration of highly invasive human breast cancer MDA-MB-231 and BT-549 cells were investigated by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) MTT and wound-healing assay. Effects of miR-200c on the expression of adhesion molecules in cells of 2 cell lines were examined by Western blot.
RESULTS: MTT assay showed that miR-200c could inhibit the proliferation of MDA-MB-231 and BT-549 cells. Wound-healing assay results showed that miR-200c could inhibit the migration of MDA-MB-231 and BT-549 cells. Western blot results showed that miR-200c up-regulated the expression of E-cadherin protein and down-regulated the expression of Vimentin protein.
CONCLUSIONS: The results showed that miR-200c can inhibit the proliferation of highly invasive human breast cancer cells and may inhibit cell migration by up-regulating the expression of E-cadherin protein and down-regulating the expression of Vimentin protein.

This article has been withdrawn. The Publisher apologizes for any inconvenience this may cause.

Free PDF Download

To cite this article

Y. Lei, Y. Ma, Y. Liu, X.-F. Wang
Effect of miR-200c on migration and proliferation of breast cancer MDA-MB-231 cells and BT-549 cells and the possible mechanism

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 2
Pages: 735-739
DOI: 10.26355/eurrev_202001_20053