The interaction between circular RNA hsa_circ_0000285 and miR-599 in thyroid cancer

D. Yang, Y. Jin, S. Cheng, Y. Yang

Department of Vascular/Thyroid Surgery, the First Hospital of China Medical University, Shenyang, China. yy.yangyu.75@163.com

 

 

 

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• Oncology

OBJECTIVE: The vital role of circular RNAs in malignant tumors has been well-studied. Thyroid cancer (TC) is one of the most ordinary malignant tumors. Regulatory effect of hsa_circ_0000285 on metastatic TC was explored in this research.

PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was performed to detect hsa_circ_0000285 expression in TC tissues. Knockdown and overexpression of Hsa_circ_0000285 models were established in TC cells. Moreover, wound healing assay and transwell assay were conducted to identify the role of hsa_circ_0000285 in regulating cellular phenotypes of TC cells. Furthermore, the interaction between hsa_circ_0000285 and miR-599 in TC cells was uncovered by the Dual-Luciferase reporter gene assay.

RESULTS: Hsa_circ_0000285 level was significantly higher in TC samples than that in adjacent samples. Migration and invasion of TC cells were reduced after silence of hsa_circ_0000285, while their metastatic abilities were enhanced by overexpression of hsa_circ_0000285. Moreover, RT-qPCR results revealed that miR-599 was downregulated via overexpression of hsa_circ_0000285, while miR-599 was upregulated via knockdown of hsa_circ_0000285. Furthermore, the Dual-Luciferase reporter gene assay showed that miR-599 was directly targeted by hsa_circ_0000285 in TC.

CONCLUSIONS: Hsa_circ_0000285 could enhance cell metastasis of TC by targeting miR-599. Hsa_circ_0000285/miR-599 may be utilized as potential therapeutic targets in TC.

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