Eur Rev Med Pharmacol Sci 2020; 24 (10): 5519-5525
DOI: 10.26355/eurrev_202005_21337

Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer

Y.-F. Xue, M. Li, W. Li, Q. Lin, B.-X. Yu, Q.-B. Zhu, H.-J. Chen

Department of Internal Medicine-Neurology, Taizhou First People’s Hospital, Taizhou, China. zqbchj@163.com


OBJECTIVE: The aim of this study was to explore the association between circ-CSPP1 and the proliferation, invasion, and migration of glioma cancer (GC).

PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to detected circ-CSPP1 expression in GC tissues and cells. Subsequently, siRNA was transfected to suppress circ-CSPP1 expression in vitro. Cell counting kit-8 (CCK-8) assay, colony formation assay, and 5-Ethynyl-2’-deoxyuridine (EdU) staining assay were performed to examine the proliferation of GC cells. Meanwhile, transwell assay was conducted to determine the invasion and migration of GC cells. Furthermore, Western blot assay was conducted to analyze the protein expressions of E-cadherin, N-cadherin, and Vimentin.

RESULTS: Circ-CSPP1 expression was significantly up-regulated both in GC tissues and cells. GC cells with low expression of circ-CSPP1 showed significantly reduced proliferation, invasion, and migration abilities. In addition, up-regulated E-cadherin protein expression, along with down-regulated N-cadherin and Vimentin protein expressions were observed in GC cells with circ-CSPP1 siRNA treatment.

CONCLUSIONS: Circ-CSPP1 promoted the proliferation, invasion, and migration of GC cells.

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To cite this article

Y.-F. Xue, M. Li, W. Li, Q. Lin, B.-X. Yu, Q.-B. Zhu, H.-J. Chen
Roles of circ-CSPP1 on the proliferation and metastasis of glioma cancer

Eur Rev Med Pharmacol Sci
Year: 2020
Vol. 24 - N. 10
Pages: 5519-5525
DOI: 10.26355/eurrev_202005_21337