MiRNA-1246 suppresses the proliferation and migration of renal cell carcinoma through targeting CXCR4

H.-T. Liu, W.-X. Fan

Department of Urology Surgery, Daqing Oilfield General Hospital, Daqing, China. fanwx1999@163.com

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• Oncology

OBJECTIVE: To uncover the role of miRNA-1246 in influencing the proliferative and migratory capacities of RCC by binding CXCR4 and downregulating its level.

PATIENTS AND METHODS: Relative levels of miRNA-1246 and CXCR4 in 40 paired RCC tissues and adjacent normal tissues were determined. The binding relationship between miRNA-1246 and CXCR4 was confirmed by Dual-Luciferase reporter assay. Proliferative and migratory abilities of RCC regulated by miRNA-1246 and CXCR4 were assessed.

RESULTS: MiRNA-1246 was downregulated in RCC tissues and cell lines. Overexpression of miRNA-1246 attenuated proliferative and migratory capacities of 786-O and 769-P cells. CXCR4 was the direct target of miRNA-1246 and its level was negatively regulated by miRNA-1246. Silence of CXCR4 inhibited RCC to proliferate and migrate.

CONCLUSIONS: MiRNA-1246 attenuates proliferative and migratory abilities of RCC by downregulating CXCR4. MiRNA-1246/CXCR4 axis could be potential therapeutic target for RCC.

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