Circ_0032627 accelerates proliferation of gastric cancer cells through regulating miR-502-5p expression

H.-F. Yin, Q. Wang, X.-T. Huang, N.-Y. Wang, M. Yuan, D. Wu, X. Sun, Y. Qin, Y.-H. Fei

Department of Oncology, Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, China. gyqjy68@sohu.com

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• Oncology

OBJECTIVE: To explore the biological functions of circ_0032627 in the progression of gastric cancer (GC).

MATERIALS AND METHODS: The expression level of circ_0032627 in GC cell lines and gastric mucosal cell lines was measured via quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Methyl thiazolyl tetrazolium (MTT) assay, colony formation assay, and FACS were performed to examine the influences of circ_0032627 on the proliferation and apoptosis of GC cells. The relationship between circ_0032627 and micro ribonucleic acids (miRNAs) was predicted on-line using StarBase software, and whether circ_0032627 can act as the sponge of the selected miRNAs was verified via Dual-Luciferase reporter assay and qRT-PCR. Finally, MTT assay was conducted to detect the influences of the co-knockdown of circ_0032627 and the selected miRNAs on the proliferation of GC cells.

RESULTS: Compared with that in gastric mucosal cell lines, the expression level of circ_0032627 was upregulated in the selected four GC cell lines, and circ_0032627 knockdown substantially inhibited the proliferation of GC cells, but promoted their apoptosis. Circ_0032627 could act as a sponge of miR-502-5p, and miR-502-3p knockdown reversed the inhibitory effect of circ_0032627 on the proliferation of GC cells.

CONCLUSIONS: The expression level of circ_0032627 is raised in GC cells, and circ_0032627 affects the proliferation and apoptosis of GC cells by sponging miR-502-5p.

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