MiRNA-146b-5p inhibits the malignant progression of gastric cancer by targeting TRAF6

J.-N. Ding, Y.-F. Zang, Y.-L. Ding

Department of Medicine, Queen Mary School, Medical College of Nanchang University, Nanchang, China. dingyinlu@126.com

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• Oncology

OBJECTIVE: MicroRNAs (miRNAs) are 22 nucleotides long that are extensively expressed in eukaryotes. They are vital regulators in pathological processes. This study aims to illustrate the role of miRNA-146b-5p in the development of gastric cancer (GC).

PATIENTS AND METHODS: MiRNA-146b-5p levels in 62 GC species and matched paracancerous ones were detected. Influences of miRNA-146b-5p level on clinical parameters of GC patients were assessed. Phenotype changes of AGS and SGC-7901 cells overexpressing miRNA-146b-5p were evaluated by Cell Counting Kit-8 (CCK-8) and transwell assay, respectively. Luciferase assay and rescue experiments were conducted to uncover the mechanism of miRNA-146b-5p in regulating the development of GC.

RESULTS: MiRNA-146b-5p was downregulated in GC species than paracancerous ones. Lower level of miRNA-146b-5p was observed in GC patients combined lymphatic metastasis and distant metastasis than those without metastases. In vitro overexpression of miRNA-146b-5p attenuated proliferative and migratory potentials of GC cells. TRAF6 was the target of miRNA-146b-5p, which was responsible for the development of GC regulated by miRNA-146b-5p.

CONCLUSIONS: MiRNA-146b-5p level is negatively correlated to lymphatic metastasis and distant metastasis rates of GC. It suppresses the malignant development of GC by targeting TRAF6.

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