The influence of oncolytic reovirus on the biological activities of umbilical cord-derived mesenchymal stem cells

X.-Y. Wang, Y.-C. Yang, N.-X. Wang, J.-W. Xu, Y.-H. Zhou, X.-J. Wu, Z.-X. He, X. Zhao

Stem Cell and Tissue Engineering Research Center, Guizhou Medical University, Guiyang, China. xingzhao@gmc.edu.cn

---

• Infectious Diseases

OBJECTIVE: To investigate the influence of oncolytic reovirus on the biological activities of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) as a novel virotherapy strategy.

MATERIALS AND METHODS: The Cell Counting Kit-8 assay was used to detect the viability of hUC-MSCs infected with different multiplicities of infection (MOIs) of reoviruses. The biological activities (proliferation, marker expression, multipotency, and migration) of hUC-MSCs were verified by assaying osteogenic and adipogenic differentiation potential, flow cytometry, and electrical cell-substrate impedance sensing, respectively.

RESULTS: The viability of hUC-MSCs slightly decreased by infection with low titers of reoviruses. A MOI of 1 had no effect on the viability of hUC-MSCs within 96 h. The biological activities (proliferation, marker expression, multipotency, and migration) of hUC-MSCs were not affected by reovirus infection at a MOI of 1.

CONCLUSIONS: Reovirus at a MOI of 1 had no effect on the biological activities of hUC-MSCs.

Free PDF Download