MiR-296-3p promotes the development and progression of preeclampsia via targeting the CEMIP

X.-S. Li, L. Liang, J. Zhang, M.-Y. Tong, C.-F. Xia, Q.-X. Yang, X.-R. Wang

Department of Obstetrics and Gynaecology, Daqing People’s Hospital, Daqing, China. j.w.b@163.com

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• Obstetrics

OBJECTIVE: Preeclampsia (PE) is one of common pregnancy diseases, which has seriously threatened the health of the gravidas. Although upregulated miR-269-3p has been found in the placentas of the patients with PE, the regulation mechanisms of miR-296-3p remain unclear.

PATIENTS AND METHODS: In this study, the placentas of the patients and normal gravidas were used to observe the difference in miR-296-3p expression level, and HTR-8/Svneo and JAR cells were used to investigate the role of miR296-3p in trophoblast cells. Besides, qRT-PCR, Western blot, CCK-8 assay, Dual-Luciferase reporter gene assay and transwell assay were used to explore the functions and regulation mechanisms of miR-296-3p on PE.

RESULTS: The results showed that miR-296-3p was upregulated in the PE-placentas, and increased miR-296-3p could inhibit the proliferation, invasion and migration of HTR-8/Svneo and JAR cells. Besides, miR-296-3p could directly target the 3’-UTR of CEMIP, and the phenomena induced by increased miR-296-3p, including decreased β-catenin and p-AKT and weakened proliferation, invasion and migration abilities, could be reversed by upregulating the expression level of CEMIP.

CONCLUSIONS: To summarize, this study suggests that miR-296-3p inactivates the Wnt/β-catenin and PI3K/AKT pathways to promote the progression of PE via targeting the CEMIP.

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