In vitro assessment of the DNA damage and senility of CD117+ stem cells collected from diabetic mice

Q.-Y. She, Y. Zhu, G. Chen, Y. Liu, C.-X. Ruan, Q. Wang, X. Sheng, B. Deng, H.-X. Liang, Y.-T. Liu, C.A. Young, S.-L. Qin

Department of Endocrinology, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, China. 1094821826@qq.com

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• Cell biology
• Diabetes

OBJECTIVE: Angiogenesis impairment is a common feature of diabetes mellitus (DM), whereas CD117+ bone marrow cells (BMCs) injury might be responsible for such complication. In this study, we studied the effect of hyperglycemia on the DNA damage and senility of CD117+ bone marrow cells.

MATERIALS AND METHODS: We isolated CD117+ BMCs from the Streptozotocin (STZ) induced diabetes and healthy control mice. Oxidative stress was detected by flow cytometric analysis. γ-H2AX, which is the DNA damage mark, was detected by using Western blotting and immunofluorescence histochemistry. We also detected the expression of γ-H2AX and p16 by using Western blotting.

RESULTS: Compared with the control mice, the level of reactive oxygen species (ROS) was increased significantly in the CD117+ BMCs collected from the diabetic mice (p<0.05), and the percentage of γ-H2AX positive cells was higher significantly (p<0.01). The expression of γ-H2AX and p16 was increased significantly in the CD117+ BMCs from the diabetic mice.

CONCLUSIONS: Our experiments demonstrated the oxidative stress in CD117+ BMCs under DM conditions, while accelerating the DNA damage and senility in CD117+ BMCs as well.

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