Eur Rev Med Pharmacol Sci 2023; 27 (6): 2277-2287
DOI: 10.26355/eurrev_202303_31761

Elucidation of the role of α-lipoic acid and vitamin C in methotrexate-induced hepatoxicity in mice

H.M. Al-kuraishy, R.R. Abd Hasan, A.I.A. Al-Gareeb, S.S. Aggad, A.S.M. Aljohani, I.M. El-Ashmawy, E. Elekhnawy, S.A. Mostafa, W.A. Negm, G.E. Batiha

Department of Clinical Pharmacology and Medicine, College of Medicine, University of Al-Mustansiriyah, Baghdad, Iraq. walaa.negm@pharm.tanta.edu.eg


OBJECTIVE: Although methotrexate (MTX) is used to treat several malignancies and chronic inflammatory diseases, its clinical use is constrained because of its negative side effects, the most prevalent of which are hepatotoxicity and nephrotoxicity. So, this study aims to determine whether α-lipoic acid (ALA) and vitamin C can protect mice against the liver damage that methotrexate causes.

MATERIALS AND METHODS: A total of 49 male mice were divided into seven groups at random. Group I received sodium bicarbonate, while groups II to VII received an intraperitoneal injection of MTX (20 mg/kg) on the tenth day, following ten days of pretreatment with ALA (60 mg/Kg), ALA (120 mg/Kg), vitamin C (100 mg/Kg), vitamin C (200 mg/Kg), ALA (60 mg/Kg), and vitamin C (100 mg/kg).

RESULTS: When compared to mice in group I, mice in group II (the control group) had significantly higher levels of the enzymes malondialdehyde (MDA), alanine transaminase (ALT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) and significantly lower (p <0.05) levels of the enzymes superoxide dismutase (SOD) and glutathione (GSH). As compared to the control group, pretreatment groups with ALA and vitamin C showed a dose-dependent substantial rise (p <0.05) in GSH and SOD levels, a dose-dependent notable decrease (p <0.05) in MDA, ALT, ALP, and LDH levels, and better liver histological architecture. In order to increase the antioxidant capacity, pretreatment with ALA and vitamin C may be able to prevent MTX-induced hepatotoxicity.

CONCLUSIONS: These results imply that ALA and vitamin C are useful in the treatment of MTX-induced liver damage.

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H.M. Al-kuraishy, R.R. Abd Hasan, A.I.A. Al-Gareeb, S.S. Aggad, A.S.M. Aljohani, I.M. El-Ashmawy, E. Elekhnawy, S.A. Mostafa, W.A. Negm, G.E. Batiha
Elucidation of the role of α-lipoic acid and vitamin C in methotrexate-induced hepatoxicity in mice

Eur Rev Med Pharmacol Sci
Year: 2023
Vol. 27 - N. 6
Pages: 2277-2287
DOI: 10.26355/eurrev_202303_31761