Genome wide gene expression analysis of macrophages from ankylosing spondylitis patients under interferon-gamma treatment

H. Ma, B. Ye, Q. Wei, X.D. Zhu

Department of Orthopaedic Surgery, Shanghai First Rehabilitation Hospital, Shanghai, P. R. China. mdzhuxiaodong@hotmail.com

 

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• Rheumatology

BACKGROUND: Ankylosing spondylitis (AS) is a common and highly heritable arthropathy, but the pathogenesis of which is poorly understood, especially the mechanisms in genomics.

AIM: Our work is aim to study the mechanisms of AS in genomics.

MATERIALS AND METHODS: we used microarray dataset GSE11886 from Gene Expression Omnibus (GEO). According to our GSEA approach on the microarray datasets related to AS, we have identified the significantly associated pathways with this disease respectively dependent and independent to the factor of interferon-gamma (IFN-γ).

RESULTS: As a result, we have identified 9 most significant pathways in the comparison of AS patients to control under none treatment, including 5 up-regulated and 4 down-regulated pathways in IFN-gamma-independent study. On the contrary, 11 most significantly up-regulated pathways such as renin-angiotensin system, O-Glycan biosynthesis and gap junction in the comparison of AS patients to control under the treatment of IFN in IFN-gamma-dependent study.

CONCLUSIONS: These may be helpful for understanding the mechanisms of AS regulation under interferon-gamma treatment in genome wide.

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