Eur Rev Med Pharmacol Sci 2019; 23 (11): 4756-4762
DOI: 10.26355/eurrev_201906_18057

Long noncoding RNA MNX1-AS1 overexpression promotes the invasion and metastasis of gastric cancer through repressing CDKN1A

J.-X. Ma, Y.-L. Yang, X.-Y. He, X.-M. Pan, Z. Wang, Y.-W. Qian

Department of General Surgery, Gansu Provincial Hospital, Lanzhou, China. majianxun68@sina.com


OBJECTIVE: Recently, long non-coding RNAs (lncRNAs) have attracted much attention for their roles in tumor progression. The aim of this study was to investigate the specific role of lncRNA MNX1-AS1 in the development of gastric cancer (GC), and to explore the underlying mechanism.

PATIENTS AND METHODS: MNX1-AS1 expression in both GC cells and tissue samples was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Moreover, the relationship between MNX1-AS1 expression and the overall survival rate of GC patients was explored. Furthermore, wound healing assay and transwell assay were conducted. In addition, the underlying mechanism of MNX1-AS1 in GC was explored by performing RT-qPCR and Western blot assay.

RESULTS: MNX1-AS1 expression in GC samples was significantly higher than that of the corresponding normal tissues. Meanwhile, MNX1-AS1 expression was associated with the overall survival time of GC patient. Moreover, the migration and invasion of GC cells were markedly promoted after MNX1-AS1 overexpression in vitro. The mRNA and protein expressions of CDKN1A were remarkably down-regulated after MNX1-AS1 overexpression. Furthermore, the expression level of CDKN1A was negatively correlated with the expression of MNX1-AS1 in GC tissues.

CONCLUSIONS: Our results suggested that MNX1-AS1 could enhance the metastasis and invasion of GC cells via suppressing CDKN1A. Furthermore, MNX1-AS1 might be a potential therapeutic target for GC.

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To cite this article

J.-X. Ma, Y.-L. Yang, X.-Y. He, X.-M. Pan, Z. Wang, Y.-W. Qian
Long noncoding RNA MNX1-AS1 overexpression promotes the invasion and metastasis of gastric cancer through repressing CDKN1A

Eur Rev Med Pharmacol Sci
Year: 2019
Vol. 23 - N. 11
Pages: 4756-4762
DOI: 10.26355/eurrev_201906_18057