Long non-coding RNA MNX1-AS1 promoted osteosarcoma proliferation and invasion via inhibiting KISS1

Y.-X. Zhang, H.-X. Cui, L. Liu, G.-K. Yi

Department of Emergency Surgery, Jining No. 1 People’s Hospital, Jining, China. ygk0113@sina.com

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• Oncology

OBJECTIVE: Recent studies revealed that long non-coding RNAs (lncRNAs) participate in the progression and development of many human diseases. In this work, we are committed to uncovering the association between lncRNA MNX1-AS1 and the development of osteosarcoma.

PATIENTS AND METHODS: MNX1-AS1 expression of osteosarcoma cells and tissue samples was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR). Besides, we conducted functional assays including cell proliferation assay, colony formation, and transwell assays. Furthermore, the underlying mechanism including RT-qPCR and Western blot assay was performed.

RESULTS: MNX1-AS1 was higher-expressed in osteosarcoma samples than that in adjacent tissues. The abilities of proliferation and invasion were suppressed after MNX1-AS1 was knocked down in vitro. Moreover, KISS1 expression was upregulated at mRNA and protein level via silence of MNX1-AS1. Furthermore, the underlying mechanism of the development of osteosarcoma were investigated by RT-qPCR and Western blot assay.

CONCLUSIONS: Our study demonstrated that MNX1-AS1 could enhance osteosarcoma cell proliferation and invasion by inhibiting KISS1, which might contribute to the therapy for osteosarcoma.

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